| Gene | Tmem50b |
| Chromosome | 16 |
| Deletion Start | 91,587,037 |
| Deletion End | 91,576,093 |
| Deletion Size | 10,945 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGCTGGCTCTAAGCTCACTATC |
| TUP | | | CCCAGCATCGTAGGTCAGCTGTAAGGG |
| TUR | | | GCTGCCCTGTGGTTCATAC |
| TDF | | | GGCTGATGCTATGACCGACAC |
| TDP | | | CTCCTGCCACTCCTTCACAGCATGG |
| TDR | | | AGAGCCTACTGAGGGACACAAC |
PCR Primers
| Primer | Sequence |
| SU | AGCCAGGATCTCAGGTATGC |
| SD | ACCTGGCAGAAATGCCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17219A-B9 | VGB6 | C57BL/6NTac | |
| 17219A-D4 | VGB6 | C57BL/6NTac | |
| 17219A-F10 | VGB6 | C57BL/6NTac | |
| 17219A-H1 | VGB6 | C57BL/6NTac | |
