| Gene | Tmem57 |
| Chromosome | 4 |
| Deletion Start | 134,853,114 |
| Deletion End | 134,828,012 |
| Deletion Size | 25,103 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,852,456 | 134,852,474 | CGCGGCTTTTCTCAGTTCC |
| TUP | 134,852,428 | 134,852,455 | TCAGCAAACATTCTGACAATTGCAAGCA |
| TUR | 134,852,392 | 134,852,414 | CAAACCTGAGTTGGGCGAGAATC |
| TDF | 134,828,171 | 134,828,189 | TGCCAGTGGAGTTGTGAAC |
| TDP | 134,828,146 | 134,828,168 | CTCGCCTCGAAGTCACAGCGCTA |
| TDR | 134,828,114 | 134,828,136 | TTTACTAGACGAGGAAGGAATGC |
PCR Primers
| Primer | Sequence |
| SU | TGTCATTTCTTTGTTTCCGAAG |
| SD | CAGGATGAACACACAGCGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17221A-D1 | VGB6 | C57BL/6NTac | |
| 17221A-E8 | VGB6 | C57BL/6NTac | |
| 17221A-E4 | VGB6 | C57BL/6NTac | |
