| Gene | Tmem63a |
| Chromosome | 1 |
| Deletion Start | 180,974,374 |
| Deletion End | 180,946,583 |
| Deletion Size | 27,792 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 180,947,452 | 180,947,472 | AGATGGGAGGGTGAAGTCCAC |
| TUP | 180,947,473 | 180,947,497 | ATCCTAGGTCTGCCTGGAGCAGGGT |
| TUR | 180,947,503 | 180,947,523 | TCCTAGCTTCCCTACCCAAAC |
| TDF | 180,974,099 | 180,974,118 | GGGCATGACCAGGATGTGAG |
| TDP | 180,974,120 | 180,974,143 | TGTCAGGCTTGCCACCATCCAAGA |
| TDR | 180,974,145 | 180,974,166 | GTCGGCAAAGGCAAAGAGTTAG |
PCR Primers
| Primer | Sequence |
| SU | AGATGACAGCTGAGGCTTCC |
| SD | CTTGGCCACAAACCACTAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17222A-C10 | VGB6 | C57BL/6NTac | |
| 17222A-C11 | VGB6 | C57BL/6NTac | |
| 17222A-D6 | VGB6 | C57BL/6NTac | |
| 17222A-G11 | VGB6 | C57BL/6NTac | |
| 17222A-F1 | VGB6 | C57BL/6NTac | |
| 17222A-D8 | VGB6 | C57BL/6NTac | |
