| Gene | Tmem63b |
| Chromosome | 17 |
| Deletion Start | 45,679,687 |
| Deletion End | 45,660,642 |
| Deletion Size | 19,046 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,679,305 | 45,679,322 | CCTTAGGCCCTGCTGTTC |
| TUP | 45,679,275 | 45,679,301 | TTCTCTATTCTCCGGAAGGTGGCCTGG |
| TUR | 45,679,227 | 45,679,249 | TCTTTCTTACCTGTCTGCATCTG |
| TDF | 45,661,165 | 45,661,184 | GGCAGTGGGAAGGCTTTGAC |
| TDP | 45,661,137 | 45,661,163 | TGCAGACTCAATCCCAGTAGTCCTCCA |
| TDR | 45,661,106 | 45,661,125 | TGCCGTGAGATCAGGAACAG |
PCR Primers
| Primer | Sequence |
| SU | CCCTCCTCATTTCTCGGG |
| SD | GTGACAACTCCTGATCTTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17223A-D3 | VGB6 | C57BL/6NTac | |
| 17223A-H2 | VGB6 | C57BL/6NTac | |
| 17223A-E6 | VGB6 | C57BL/6NTac | |
| 17223A-G12 | VGB6 | C57BL/6NTac | |
| 17223A-F1 | VGB6 | C57BL/6NTac | |
| 17223A-D6 | VGB6 | C57BL/6NTac | |
