| Gene | Tmprss6 |
| Chromosome | 15 |
| Deletion Start | 78,465,340 |
| Deletion End | 78,440,214 |
| Deletion Size | 25,127 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 78,464,602 | 78,464,622 | CCCAGGCTTGTGAACCTCTTC |
| TUP | 78,464,576 | 78,464,600 | TCATATCTGACTTGGGCCCTTTGGC |
| TUR | 78,464,500 | 78,464,521 | AGGGTGTCTCTGGGTCCAGAAC |
| TDF | 78,441,022 | 78,441,042 | GCTGGACTGGTGGATGCTATC |
| TDP | 78,440,995 | 78,441,020 | CCAGGACAAGATCTGGACTGAGCAGA |
| TDR | 78,440,965 | 78,440,987 | TCACTGTGCTCTTGACTGCTTAG |
PCR Primers
| Primer | Sequence |
| SU | TGGGCTTTACCTGCTTCATC |
| SD | GGCACCTTCTTTCTCTGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17226A-B11 | VGB6 | C57BL/6NTac | |
| 17226A-E8 | VGB6 | C57BL/6NTac | |
| 17226A-H2 | VGB6 | C57BL/6NTac | |
| 17226A-F7 | VGB6 | C57BL/6NTac | |
| 17226A-A12 | VGB6 | C57BL/6NTac | |
