| Gene | Car4 |
| Chromosome | 11 |
| Deletion Start | 84,965,822 |
| Deletion End | 84,957,890 |
| Deletion Size | 7,933 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,958,300 | 84,958,320 | GCAGCAGGTTGGATGTATTGG |
| TUP | 84,958,323 | 84,958,347 | TCCCGGTGTTCCCTTCTTCTGACTC |
| TUR | 84,958,352 | 84,958,370 | GAAGCTCAGAGGGCAGGAC |
| TDF | 84,965,202 | 84,965,223 | CTCTGGGAAGACAGCAGGAAAC |
| TDP | 84,965,224 | 84,965,249 | CCAGAGGACCAAACTCCCTTGGGCTT |
| TDR | 84,965,260 | 84,965,278 | GGTGACACCACACTGAGAC |
PCR Primers
| Primer | Sequence |
| SU | TACTCCGCTTCTAAGCCCTG |
| SD | GTAGGGTGATTCATGCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17228A-C10 | VGB6 | C57BL/6NTac | |
| 17228A-D2 | VGB6 | C57BL/6NTac | |
| 17228A-G8 | VGB6 | C57BL/6NTac | |
| 17228A-F9 | VGB6 | C57BL/6NTac | |
| 17228A-F4 | VGB6 | C57BL/6NTac | |
