| Gene | Cln6 |
| Chromosome | 9 |
| Deletion Start | 62,850,932 |
| Deletion End | 62,838,884 |
| Deletion Size | 12,049 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 62,839,414 | 62,839,433 | TGAGCCAAAGCAGGTGACAG |
| TUP | 62,839,438 | 62,839,464 | TTCTGGAGGGCTTTCTGGAGTTGAACA |
| TUR | 62,839,489 | 62,839,508 | ACCATGCCCGGCAGTAAGTG |
| TDF | 62,850,722 | 62,850,745 | CAACGGCCTCTTCCTCTTGTATTC |
| TDP | 62,850,746 | 62,850,768 | CTTCGCGCTCACCCTCTCACTGG |
| TDR | 62,850,774 | 62,850,792 | ACAGCCAAGCCACCCACAG |
PCR Primers
| Primer | Sequence |
| SU | ACCTCTGGGACATAAGTTAGG |
| SD | GAAGCCCAGAGATGGAACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17229A-C1 | VGB6 | C57BL/6NTac | |
| 17229A-F9 | VGB6 | C57BL/6NTac | |
| 17229A-F8 | VGB6 | C57BL/6NTac | |
| 17229A-E6 | VGB6 | C57BL/6NTac | |
