| Gene | Dhrs7b |
| Chromosome | 11 |
| Deletion Start | 60,857,949 |
| Deletion End | 60,830,715 |
| Deletion Size | 27,235 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,831,466 | 60,831,484 | CAACAGCCAGCCTCCAGAC |
| TUP | 60,831,486 | 60,831,511 | CAGCTGCTAAGACCCTGTAGGCACTG |
| TUR | 60,831,522 | 60,831,542 | GGGAAGCCAGAGCCAACTAAC |
| TDF | 60,857,278 | 60,857,296 | GCCTGGAGCCCACTTTGAG |
| TDP | 60,857,298 | 60,857,319 | CCTCCCTGTGCTGGGTTTCGGC |
| TDR | 60,857,326 | 60,857,347 | TGTGTCTGTTTGGTGCTCTGTG |
PCR Primers
| Primer | Sequence |
| SU | GTAACAACATCACATCGCCG |
| SD | GGTAGGATGAATAGGGATGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17230A-B6 | VGB6 | C57BL/6NTac | |
| 17230A-G7 | VGB6 | C57BL/6NTac | |
| 17230A-D7 | VGB6 | C57BL/6NTac | |
| 17230A-D1 | VGB6 | C57BL/6NTac | |
