| Gene | Gpc4 |
| Chromosome | X |
| Deletion Start | 52,091,795 |
| Deletion End | 52,053,482 |
| Deletion Size | 38,314 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GGTCAGCGAACAGTGCAAC |
| TUP | | | TTGCAAGCCATCTTTGCATCCCGT |
| TUR | | | AACTGTCAGCTCTTCCATGTAAC |
| TDF | | | GCCAACCAGGGCAACAAC |
| TDP | | | CCAGAAGTCCAGGTTGACACCAGCA |
| TDR | | | CCGAAGGGCCATGATCTGAC |
PCR Primers
| Primer | Sequence |
| SU | CCATGAGATCTTTCCCTTGTG |
| SD | TGCCAAGAAAGGACCCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17231C-B8 | VGB6 | C57BL/6NTac | |
| 17231C-F10 | VGB6 | C57BL/6NTac | |
| 17231C-H4 | VGB6 | C57BL/6NTac | |
