| Gene | Zdhhc1 |
| Chromosome | 8 |
| Deletion Start | 105,483,780 |
| Deletion End | 105,472,589 |
| Deletion Size | 11,192 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,483,450 | 105,483,472 | AGCTGGGTGCATGTGAGGTAGAG |
| TUP | 105,483,422 | 105,483,449 | CAGGGTTGACTCAGTTGATTCTCTGCCA |
| TUR | 105,483,391 | 105,483,413 | CAGAGGAAGGGTCACGTTACAAG |
| TDF | 105,473,184 | 105,473,203 | AGGAGGGACTGGGTTTGGAC |
| TDP | 105,473,159 | 105,473,182 | TCTGCCCAAGAAGAGGGACATGGA |
| TDR | 105,473,122 | 105,473,141 | AAGCAGCAGAGGCGCTCATC |
PCR Primers
| Primer | Sequence |
| SU | TATGGCTGCAGAGACAGGAC |
| SD | AATGGGAGAAGGGACAGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17235A-B8 | VGB6 | C57BL/6NTac | |
| 17235A-F8 | VGB6 | C57BL/6NTac | |
| 17235A-D6 | VGB6 | C57BL/6NTac | |
| 17235A-D8 | VGB6 | C57BL/6NTac | |
| 17235A-D4 | VGB6 | C57BL/6NTac | |
| 17235A-D1 | VGB6 | C57BL/6NTac | |
