| Gene | Zdhhc3 |
| Chromosome | 9 |
| Deletion Start | 123,100,565 |
| Deletion End | 123,078,141 |
| Deletion Size | 22,425 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 123,100,500 | 123,100,520 | GAGTACCTCCAGCCAGAGAAG |
| TUP | 123,100,469 | 123,100,491 | ACCTCCCTTCCCTGGTCCTGCGG |
| TUR | 123,100,425 | 123,100,443 | GGCACAAGCAATGCCACAG |
| TDF | 123,078,593 | 123,078,612 | ACCCAGCAAGGACTCAGAAC |
| TDP | 123,078,561 | 123,078,589 | ACAGTGTCAATCTTGTTGGCAACAGTGGC |
| TDR | 123,078,522 | 123,078,540 | CGAGGGTGAACGGACAGTG |
PCR Primers
| Primer | Sequence |
| SU | TTCAGTGCTCCCGGATTTAG |
| SD | AAGAGGTTCTGAGTCCTGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17236A-G4 | VGB6 | C57BL/6NTac | |
| 17236A-E2 | VGB6 | C57BL/6NTac | |
| 17236A-A5 | VGB6 | C57BL/6NTac | |
