| Gene | Atp6v0a4 |
| Chromosome | 6 |
| Deletion Start | 38,094,051 |
| Deletion End | 38,071,854 |
| Deletion Size | 22,198 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,093,229 | 38,093,247 | GGTGCTCACGAGGCCATAG |
| TUP | 38,093,203 | 38,093,225 | TTGGGTCCGCTGAGTTACATGGC |
| TUR | 38,093,151 | 38,093,175 | GTGCTTGTTAGTCTTGCTTGTTAGG |
| TDF | 38,072,403 | 38,072,423 | GCTTTCCCTAGCAGATGTGAC |
| TDP | 38,072,372 | 38,072,398 | AGAAGCCCAACTGTGCTTTGTCTCTGT |
| TDR | 38,072,318 | 38,072,336 | CGCTGGCCTCTGTCCTATC |
PCR Primers
| Primer | Sequence |
| SU | TTTGGGTTTGATGATGTTGG |
| SD | CACAACCAGTTCAGCTACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 106 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17238A-B3 | VGB6 | C57BL/6NTac | |
| 17238A-H3 | VGB6 | C57BL/6NTac | |
