| Gene | Cfp |
| Chromosome | X |
| Deletion Start | 20,931,451 |
| Deletion End | 20,925,592 |
| Deletion Size | 5,860 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,931,129 | 20,931,147 | GGAGGAACCTGGGCTGAAC |
| TUP | 20,931,096 | 20,931,123 | AACCTAGAACTGTACTCGTTGCCCTGGA |
| TUR | 20,931,072 | 20,931,091 | GGAGGAGGTGGCACTCTTAG |
| TDF | 20,925,777 | 20,925,796 | TGAGCTGCTCTCCTCCATTG |
| TDP | 20,925,743 | 20,925,772 | CCCAATACTCCAGGCCTACAGTTTCAATGG |
| TDR | 20,925,717 | 20,925,740 | AACATTCTTCTCACCCTGACCTTC |
PCR Primers
| Primer | Sequence |
| SU | TGATTTCTGGGTAAGGTGGG |
| SD | TCTGGTAAGTGGGTGGGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17240A-B11 | VGB6 | C57BL/6NTac | |
| 17240A-D5 | VGB6 | C57BL/6NTac | |
| 17240A-D12 | VGB6 | C57BL/6NTac | |
| 17240A-F10 | VGB6 | C57BL/6NTac | |
| 17240A-H6 | VGB6 | C57BL/6NTac | |
