| Gene | Ctsd |
| Chromosome | 7 |
| Deletion Start | 142,387,801 |
| Deletion End | 142,376,532 |
| Deletion Size | 11,270 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 142,386,913 | 142,386,932 | TGGAGCCATTGTAGGGAGTG |
| TUP | 142,386,885 | 142,386,910 | CTAGGCTTGATCCTGCCCTGGGCTTG |
| TUR | 142,386,833 | 142,386,853 | TGGAGTCCACAACACCTCTTC |
| TDF | 142,377,371 | 142,377,390 | GGGAGCTGTCCTACCTGAAC |
| TDP | 142,377,346 | 142,377,369 | TCACTCGAAAGGCCTACTGGCAGG |
| TDR | 142,377,316 | 142,377,334 | CGACACCCACGACTCACTG |
PCR Primers
| Primer | Sequence |
| SU | AGACAAAGACCACATCCTGG |
| SD | AGTAGAACGTGGTGCCATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17241A-A10 | VGB6 | C57BL/6NTac | |
| 17241A-H1 | VGB6 | C57BL/6NTac | |
| 17241A-C1 | VGB6 | C57BL/6NTac | |
| 17241A-D7 | VGB6 | C57BL/6NTac | |
