| Gene | Cyba |
| Chromosome | 8 |
| Deletion Start | 122,432,864 |
| Deletion End | 122,424,857 |
| Deletion Size | 8,008 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,432,278 | 122,432,298 | CCGAGTTAAGATGCAGGACAC |
| TUP | 122,432,249 | 122,432,274 | TCTTTAGTCAGGTTGACACTGGCCGT |
| TUR | 122,432,213 | 122,432,232 | TGGCAAACCTCCCAGACAAG |
| TDF | 122,425,065 | 122,425,083 | CCATCCGAGGTGAGCAGTG |
| TDP | 122,425,036 | 122,425,061 | TCCCATTGAGCCTAAACCCAAGGAGC |
| TDR | 122,425,014 | 122,425,032 | ATGGTGCCTCCAACCTGTG |
PCR Primers
| Primer | Sequence |
| SU | AGCCTTGCACACTATGGTCC |
| SD | AGCACCCTGTACTCTGGCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17242A-B10 | VGB6 | C57BL/6NTac | |
| 17242A-C2 | VGB6 | C57BL/6NTac | |
| 17242A-F2 | VGB6 | C57BL/6NTac | |
| 17242A-G1 | VGB6 | C57BL/6NTac | |
| 17242A-E4 | VGB6 | C57BL/6NTac | |
| 17242A-D7 | VGB6 | C57BL/6NTac | |
