| Gene | Pdcd1 |
| Chromosome | 1 |
| Deletion Start | 94,052,487 |
| Deletion End | 94,039,272 |
| Deletion Size | 13,216 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,051,754 | 94,051,772 | GGACTGAGCCAACCTTCAC |
| TUP | 94,051,732 | 94,051,753 | TGAGAACCTGGTCCTGGGACCA |
| TUR | 94,051,701 | 94,051,724 | TGTTGACATCCAGGAGAAACAAAG |
| TDF | 94,040,197 | 94,040,221 | GAGAGAGAAAGATGGAGAGACAGAG |
| TDP | 94,040,167 | 94,040,194 | AGGACATTCCTCCACTAGGGAAGATGGC |
| TDR | 94,040,146 | 94,040,165 | GGGTTAGAGGGCAGCTACAG |
PCR Primers
| Primer | Sequence |
| SU | ACCTCTAGTTGCCTGTTCTC |
| SD | GTATCATCTCCCAAGGGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17244A-B12 | VGB6 | C57BL/6NTac | |
| 17244A-G10 | VGB6 | C57BL/6NTac | |
