| Gene | Rnf24 |
| Chromosome | 2 |
| Deletion Start | 131,313,302 |
| Deletion End | 131,303,405 |
| Deletion Size | 9,898 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 131,312,494 | 131,312,516 | GCATTCACAGAGGCATGGGATAG |
| TUP | 131,312,472 | 131,312,493 | ACGAGGTCAGCTCACGTGGCGT |
| TUR | 131,312,430 | 131,312,452 | GTCAGCCAAGCATGAAACATTAC |
| TDF | 131,304,071 | 131,304,092 | ACCAGGGAAGTGGAACATGAAC |
| TDP | 131,304,048 | 131,304,070 | ACCACTGCAGGCTTGGGCAGAGA |
| TDR | 131,304,019 | 131,304,042 | GTGCTGTATAGAGGTGTAGGATGG |
PCR Primers
| Primer | Sequence |
| SU | TGACATTTCATGTAGCACCTTATG |
| SD | TGCACAGCAGCTTACAGGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17245A-H6 | VGB6 | C57BL/6NTac | |
| 17245A-C2 | VGB6 | C57BL/6NTac | |
| 17245A-A9 | VGB6 | C57BL/6NTac | |
| 17245A-B5 | VGB6 | C57BL/6NTac | |
| 17245A-E12 | VGB6 | C57BL/6NTac | |
