| Gene | Soat2 |
| Chromosome | 15 |
| Deletion Start | 102,162,977 |
| Deletion End | 102,150,637 |
| Deletion Size | 12,341 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,151,257 | 102,151,275 | TGTGATGGCAACTGGTGTG |
| TUP | 102,151,299 | 102,151,328 | CAGCTGTAATCCTAGCACTTGGGAAGTAGA |
| TUR | 102,151,345 | 102,151,363 | GCCAAGGGTGACCTCAAAC |
| TDF | 102,162,603 | 102,162,624 | CATCCTGATGTGGACCTTTCTC |
| TDP | 102,162,625 | 102,162,644 | TTCATGGGCCAGGGCATCCA |
| TDR | 102,162,661 | 102,162,680 | CGACGAGCATACCACTCCTG |
PCR Primers
| Primer | Sequence |
| SU | TCCCTGACCTTCAGTCTTGG |
| SD | ACGTGTCAGACTTTGGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17246A-B4 | VGB6 | C57BL/6NTac | |
| 17246A-C6 | VGB6 | C57BL/6NTac | |
| 17246A-D7 | VGB6 | C57BL/6NTac | |
| 17246A-C11 | VGB6 | C57BL/6NTac | |
| 17246A-A3 | VGB6 | C57BL/6NTac | |
