17247   Gja5     Status: ES cell colonies screened / QC positives

GeneGja5
Chromosome3
Deletion Start97,050,632
Deletion End97,051,728
Deletion Size1,097
Genome Build38
MGI IDMGI:95716
NCBI Gene ID0
BAC ID411O21
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 3

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF97,051,03297,051,054GCTGGAAAGAAGTAGATGGGAAG
TUP97,051,05697,051,080TTGTCCTCCAGGGCACCCTACTCAA
TUR97,051,09697,051,114GGTGGTGCGGATCAGAATG
TDF97,051,27097,051,289GGCTGGACTGTCTCTGTTTC
TDP97,051,29197,051,315CAGCCTGGCTGAACTCTACCACCTG
TDR97,051,34597,051,363CACACCCTGCCGTGACTTG

PCR Primers

PrimerSequence
SUAACACATTTGGTGTGGATCAG
SDATGGCACATGGAAACAACTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR83 bp++-
TDFTDR94 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17247A-F4VGB6C57BL/6NTac
17247A-F7VGB6C57BL/6NTac
17247A-H3VGB6C57BL/6NTac
17247A-G6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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