| Gene | Mst1r |
| Chromosome | 9 |
| Deletion Start | 107,911,701 |
| Deletion End | 107,907,148 |
| Deletion Size | 4,554 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,907,961 | 107,907,980 | CCTCCCAGTGCCTTGCATAC |
| TUP | 107,907,981 | 107,908,005 | ACGTCTGGTCCGGCTCAATGCTGTA |
| TUR | 107,908,012 | 107,908,032 | CTCCCGGTAGTCACCAATCTC |
| TDF | 107,911,588 | 107,911,606 | CACCCGTGTGGACCTCTTC |
| TDP | 107,911,612 | 107,911,639 | ACTGTTAGGATCAGTGAAGGTCACCGCA |
| TDR | 107,911,664 | 107,911,683 | CCCATGTGGGCCACTGTAAC |
PCR Primers
| Primer | Sequence |
| SU | CCTCCCTTCGACTTTGATCC |
| SD | CCATTACCCTGAACCCTAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17258A-A10 | VGB6 | C57BL/6NTac | |
| 17258A-B1 | VGB6 | C57BL/6NTac | |
| 17258A-E8 | VGB6 | C57BL/6NTac | |
| 17258A-G2 | VGB6 | C57BL/6NTac | |
| 17258A-D5 | VGB6 | C57BL/6NTac | |
