| Gene | Klhl8 |
| Chromosome | 5 |
| Deletion Start | 103,886,146 |
| Deletion End | 103,863,059 |
| Deletion Size | 23,088 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,885,732 | 103,885,751 | TTAGCCTCAGACTCACGTTC |
| TUP | 103,885,707 | 103,885,731 | TGCCTCACGTCTCCTGAGTGTTAGG |
| TUR | 103,885,687 | 103,885,706 | GTGCAGCACATGCCTGTAAC |
| TDF | 103,863,206 | 103,863,226 | TGCTCTGTGGTGACAGTCTTG |
| TDP | 103,863,183 | 103,863,205 | TCCTGTCTCTCCAGGTGGGAGCT |
| TDR | 103,863,154 | 103,863,172 | CAGCTCGGCAGTGAGACAC |
PCR Primers
| Primer | Sequence |
| SU | TGCCGAGTCCACAAAGAAAG |
| SD | AATGATCCTCAAGGGAGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17269A-A11 | VGB6 | C57BL/6NTac | |
| 17269A-B10 | VGB6 | C57BL/6NTac | |
| 17269A-H3 | VGB6 | C57BL/6NTac | |
| 17269A-F2 | VGB6 | C57BL/6NTac | |
| 17269A-G3 | VGB6 | C57BL/6NTac | |
| 17269A-E8 | VGB6 | C57BL/6NTac | |
