| Gene | Kcnj1 |
| Chromosome | 9 |
| Deletion Start | 32,397,501 |
| Deletion End | 32,396,345 |
| Deletion Size | 1,157 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,396,530 | 32,396,551 | CGTGTTCATCACAGCCTTCTTG |
| TUP | 32,396,558 | 32,396,587 | TGGTTTCTCTTTGGTCTCCTGTGGTATGTC |
| TUR | 32,396,620 | 32,396,638 | ACGGTTGTCAGGTGGGTAG |
| TDF | 32,397,279 | 32,397,301 | CCAAGGAAGGGAAATACCGAGTG |
| TDP | 32,397,307 | 32,397,336 | CCATAACTTTGGTAAGACGGTGGAAGTGGA |
| TDR | 32,397,342 | 32,397,363 | TAGAGGCACATGGCACAATGAG |
PCR Primers
| Primer | Sequence |
| SU | GCACAGGTTATAATGGGTCACTC |
| SD | TTTCCATCAACACTGTACGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17273A-H4 | VGB6 | C57BL/6NTac | |
| 17273A-A3 | VGB6 | C57BL/6NTac | |
| 17273A-A10 | VGB6 | C57BL/6NTac | |
| 17273A-G9 | VGB6 | C57BL/6NTac | |
| 17273A-A2 | VGB6 | C57BL/6NTac | |
