| Gene | Vnn3 |
| Chromosome | 10 |
| Deletion Start | 23,904,719 |
| Deletion End | 23,851,587 |
| Deletion Size | 53,133 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,851,993 | 23,852,013 | ACCAGACCCTGAAGTGAACTG |
| TUP | 23,852,017 | 23,852,043 | TCCCTGTAGAGACCCTAGGAGGTAACA |
| TUR | 23,852,094 | 23,852,113 | GCCTGGTGACATTACTGCTC |
| TDF | 23,904,538 | 23,904,559 | GGGCTCTTTGGGAGGTTGTATG |
| TDP | 23,904,563 | 23,904,588 | AGGACTGGGCATCCAATGCTTCCTCA |
| TDR | 23,904,589 | 23,904,609 | CAGCGAGTGTGCTATGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | CTCAACTGGTTGGTTCAGTG |
| SD | CTTATGATGGTTTGAAGTGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 121 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17276A-E10 | VGB6 | C57BL/6NTac | |
| 17276A-H4 | VGB6 | C57BL/6NTac | |
| 17276A-C9 | VGB6 | C57BL/6NTac | |
