| Gene | Aldh3a2 |
| Chromosome | 11 |
| Deletion Start | 61,267,003 |
| Deletion End | 61,246,307 |
| Deletion Size | 20,697 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,266,884 | 61,266,904 | CAAGAGCGCGAGAAGGAAATC |
| TUP | 61,266,863 | 61,266,883 | TTAGCAGCCATCGCGGCAGAC |
| TUR | 61,266,838 | 61,266,857 | CCCTGTGGAGGCTCACTTTG |
| TDF | 61,246,584 | 61,246,604 | GTCATGCCAATGCTTGGACTC |
| TDP | 61,246,558 | 61,246,581 | ACCACAGTGCCGGAATAGTGCACA |
| TDR | 61,246,533 | 61,246,554 | TGCAAAGCAGCAGACATTACAG |
PCR Primers
| Primer | Sequence |
| SU | CTGAAGCCAGAAGGACTTC |
| SD | GTAGCTGGGAGGACCTAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17277A-A9 | VGB6 | C57BL/6NTac | |
| 17277A-F2 | VGB6 | C57BL/6NTac | |
| 17277A-G1 | VGB6 | C57BL/6NTac | |
| 17277A-G4 | VGB6 | C57BL/6NTac | |
| 17277A-H2 | VGB6 | C57BL/6NTac | |
| 17277A-E4 | VGB6 | C57BL/6NTac | |
