17281   Nsun5     Status: ES cell colonies screened / QC positives

GeneNsun5
Chromosome5
Deletion Start135,376,038
Deletion End135,369,987
Deletion Size6,052
Genome Build38
MGI IDMGI:2140844
NCBI Gene ID100609
BAC ID98B10
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,370,344135,370,366CGTGGCTCTCACACTTCTTTCTG
TUP135,370,372135,370,398CCAGTTGAGCTGGGTCAGATGTGACTC
TUR135,370,408135,370,430CCTACTTAGCCCTTCCTGCAATG
TDF135,375,943135,375,961CGCAAAGCAGCAGTTGGTG
TDP135,375,964135,375,988AGCATGCAGCCAAGCACATAGGCAC
TDR135,376,017135,376,038AGAGCAGGGTTCCCACAGTAAG

PCR Primers

PrimerSequence
SUCTGAATTCTAAAAAGAAAGTAGAC
SDTGTGCCTCAGGTAAGGAAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR87 bp++-
TDFTDR96 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17281A-E10VGB6C57BL/6NTac
17281A-C3VGB6C57BL/6NTac
17281A-G5VGB6C57BL/6NTac
17281A-G6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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