| Gene | 2810432D09Rik |
| Chromosome | 4 |
| Deletion Start | 41,712,770 |
| Deletion End | 41,712,235 |
| Deletion Size | 536 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 41,712,725 | 41,712,744 | AGAGCTTCCAGCCTCATCAC |
| TUP | 41,712,694 | 41,712,718 | CCCAGCTACCTCCTGACACTCTGGA |
| TUR | 41,712,670 | 41,712,688 | TTGCTCCCATCTCGGACTC |
| TDF | 41,712,338 | 41,712,356 | CCTGGCCTGGGTTCCATAC |
| TDP | 41,712,317 | 41,712,337 | CTAGTCCCAGCTGTGGCCCCA |
| TDR | 41,712,260 | 41,712,277 | AACAGTCAGGCGCAGCAG |
PCR Primers
| Primer | Sequence |
| SU | AGTCCTTGCAGCCTTGTGAG |
| SD | TTGATACCAGACCCGTCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17284A-A1 | VGB6 | C57BL/6NTac | |
| 17284A-A2 | VGB6 | C57BL/6NTac | |
| 17284A-B5 | VGB6 | C57BL/6NTac | |
| 17284A-E6 | VGB6 | C57BL/6NTac | |
| 17284A-B12 | VGB6 | C57BL/6NTac | |
