| Gene | 3110001I22Rik |
| Chromosome | 16 |
| Deletion Start | 13,677,977 |
| Deletion End | 13,677,042 |
| Deletion Size | 936 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,677,063 | 13,677,081 | GGTCACCGTCCAGGATCAG |
| TUP | 13,677,082 | 13,677,111 | TGTACAGAAGAGACAAGATGTGGCCTAACA |
| TUR | 13,677,172 | 13,677,192 | GGCTTGCAGAAAGATCACCTC |
| TDF | 13,677,759 | 13,677,782 | CGCCGTAAAGCAATAGTGTCAAAG |
| TDP | 13,677,794 | 13,677,823 | TGAACAAGCCTACTACCAAGACTGTGAGAC |
| TDR | 13,677,829 | 13,677,852 | CAACCAGCATTTTCACCACCATTC |
PCR Primers
| Primer | Sequence |
| SU | AGTATGGATCCTCTTGGCCC |
| SD | AAACCACAACTCGAATCCTAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 130 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17285A-A8 | VGB6 | C57BL/6NTac | |
| 17285A-H11 | VGB6 | C57BL/6NTac | |
| 17285A-H3 | VGB6 | C57BL/6NTac | |
| 17285A-E9 | VGB6 | C57BL/6NTac | |
| 17285A-H7 | VGB6 | C57BL/6NTac | |
| 17285A-B3 | VGB6 | C57BL/6NTac | |
