| Gene | 4921504E06Rik |
| Chromosome | 2 |
| Deletion Start | 19,553,877 |
| Deletion End | 19,533,766 |
| Deletion Size | 20,112 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,553,647 | 19,553,669 | CAGGTCTGGGTTCTAATTTCGTC |
| TUP | 19,553,623 | 19,553,646 | TGACTTCTCACCGGGTCCTATGCC |
| TUR | 19,553,554 | 19,553,573 | AACAGGCCCTGTGGTCTATG |
| TDF | 19,533,910 | 19,533,934 | TGTTGGAAGGTGAATTGTTGCTATG |
| TDP | 19,533,885 | 19,533,907 | TTCTTCCCTGGGAAGTGTGGGCT |
| TDR | 19,533,852 | 19,533,874 | AAATGCAGTAAGCACCACTACAC |
PCR Primers
| Primer | Sequence |
| SU | AGCCTTGGAGGAGAAGGAAG |
| SD | GTTGCTTCTTCACCAAGGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 116 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17286A-H9 | VGB6 | C57BL/6NTac | |
