|
|
Sequence Downloads and Strategy Information
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,143,986 | 88,144,007 | CTACGTTATGCGGTCCAGACTC |
| TUP | 88,143,962 | 88,143,984 | ATGCTCCTGGCCCTGCAGCTGTC |
| TUR | 88,143,925 | 88,143,949 | CCATAGTACACTGACCTGTTTGTTG |
| TDF | 88,115,381 | 88,115,400 | CCCATCCTCCTGTGCATTTC |
| TDP | 88,115,356 | 88,115,380 | TCACACTGGACCTGTGCTAGTCCTG |
| TDR | 88,115,296 | 88,115,318 | TCAGAACCTGCAATCAATGTTTC |
PCR Primers
| Primer | Sequence |
| SU | GGAACAATACACTCCCACCC |
| SD | CTGTTGCTGTCTTGCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the KOMP Repository.| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17287A-D3 | VGB6 | C57BL/6NTac | |
| 17287A-C4 | VGB6 | C57BL/6NTac |
