| Gene | 4930578N16Rik |
| Chromosome | 2 |
| Deletion Start | 69,789,460 |
| Deletion End | 69,758,126 |
| Deletion Size | 31,335 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 69,789,304 | 69,789,326 | TGAAATGGGCTTTAACCAAGTCG |
| TUP | 69,789,277 | 69,789,301 | AGCAGTCCCAGCAAGCATCCTTGGA |
| TUR | 69,789,257 | 69,789,275 | GAAGGCCAACCTGCCTAAC |
| TDF | 69,758,252 | 69,758,272 | GACGTGGACCACCCTTTGTAG |
| TDP | 69,758,220 | 69,758,244 | TGGGATAAGGCCAAGCTATCAGGCA |
| TDR | 69,758,134 | 69,758,156 | CCTAGCCTTCTCTTCGACTTCTG |
PCR Primers
| Primer | Sequence |
| SU | TCTCGTGCCTTACGCAGC |
| SD | GAAACTGACATTGTGTGACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 139 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17288A-B5 | VGB6 | C57BL/6NTac | |
| 17288A-H1 | VGB6 | C57BL/6NTac | |
| 17288A-G9 | VGB6 | C57BL/6NTac | |
| 17288A-G5 | VGB6 | C57BL/6NTac | |
| 17288A-G2 | VGB6 | C57BL/6NTac | |
