| Gene | 5730494M16Rik |
| Chromosome | 18 |
| Deletion Start | 25,151,269 |
| Deletion End | 25,138,836 |
| Deletion Size | 12,434 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,150,850 | 25,150,867 | GGGACCCGCAAACACTAC |
| TUP | 25,150,822 | 25,150,848 | TCTTAGCTTTCCTGAAACGGCTGACCA |
| TUR | 25,150,785 | 25,150,805 | TGCACAATGGTGAGAATGAGG |
| TDF | 25,139,605 | 25,139,624 | CAGCACACCGCTTCCCATAG |
| TDP | 25,139,578 | 25,139,603 | AAAGTTCTGCACGGACCTCTGTTGCA |
| TDR | 25,139,530 | 25,139,550 | GACACTGTCCTGCTTGCCATC |
PCR Primers
| Primer | Sequence |
| SU | CTAGGTTTTATCTGGACACAC |
| SD | GGTGCATAGCTGAAGTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17289A-A12 | VGB6 | C57BL/6NTac | |
| 17289A-G8 | VGB6 | C57BL/6NTac | |
| 17289A-C8 | VGB6 | C57BL/6NTac | |
| 17289A-E6 | VGB6 | C57BL/6NTac | |
| 17289A-B9 | VGB6 | C57BL/6NTac | |
| 17289A-H3 | VGB6 | C57BL/6NTac | |
