| Gene | 9130017N09Rik |
| Chromosome | 10 |
| Deletion Start | 79,830,130 |
| Deletion End | 79,825,754 |
| Deletion Size | 4,377 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,826,177 | 79,826,196 | GCCAGACAACCTCAGTCCAC |
| TUP | 79,826,199 | 79,826,224 | TCACAGAGGAGATCTTGGTGGACACG |
| TUR | 79,826,234 | 79,826,254 | CTGTCGAGCAGCCAGGAAATC |
| TDF | 79,829,165 | 79,829,184 | TGTCAGCCAGAGGTTCCAAC |
| TDP | 79,829,188 | 79,829,213 | TGGAATGCCAGCTCAGTGTGGCAAAT |
| TDR | 79,829,227 | 79,829,249 | CCTTCCCAGAGTCTCATGTTCAC |
PCR Primers
| Primer | Sequence |
| SU | CAGAAGTTTGTTGTGTGATCTG |
| SD | AAGATGAATGGGCCAGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17290A-A9 | VGB6 | C57BL/6NTac | |
| 17290A-A12 | VGB6 | C57BL/6NTac | |
| 17290A-B10 | VGB6 | C57BL/6NTac | |
| 17290A-C10 | VGB6 | C57BL/6NTac | |
| 17290A-H10 | VGB6 | C57BL/6NTac | |
