17292   A730011L01Rik     Status: ES cell colonies screened / QC positives

GeneA730011L01Rik
Chromosome11
Deletion Start119,507,501
Deletion End119,499,551
Deletion Size7,951
Genome Build38
MGI IDMGI:2444688
NCBI Gene ID338371
BAC ID163P6
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF119,500,451119,500,471TGACACTGGTGCTTTCAGGAC
TUP119,500,472119,500,496AGAGGGCCCATTCACAGTGCAGTCA
TUR119,500,530119,500,547TGGGAGCCAGCCACAATC
TDF119,506,602119,506,620TCCCTCGAATGCCTGTCTC
TDP119,506,621119,506,647CCATCACAGAAGTTCAGTCCTTCGAGC
TDR119,506,691119,506,709TCATGCCAGTCCTGTGTCC

PCR Primers

PrimerSequence
SUGCTCCTCATACACTCCAGGC
SDGATTGAGGCTTTCCCAACTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR97 bp++-
TDFTDR108 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17292A-E1VGB6C57BL/6NTac
17292A-F10VGB6C57BL/6NTac
17292A-G11VGB6C57BL/6NTac
17292A-H12VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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