| Gene | Abce1 |
| Chromosome | 8 |
| Deletion Start | 79,708,522 |
| Deletion End | 79,685,233 |
| Deletion Size | 23,290 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,708,184 | 79,708,206 | TCAAGCCAAAGTTCACTGTACTC |
| TUP | 79,708,157 | 79,708,181 | TGCAGTCCTGCAGATAGGTGAGTCA |
| TUR | 79,708,130 | 79,708,148 | TGGAAGGACCCTGGCAATC |
| TDF | 79,685,347 | 79,685,368 | TGCATTAAAGCGTCCCACAGAG |
| TDP | 79,685,313 | 79,685,339 | TCTAGTAGAGCAGCCCTGCATAGAGGA |
| TDR | 79,685,289 | 79,685,312 | GAGAAGTGACACAGTGAGCATTTG |
PCR Primers
| Primer | Sequence |
| SU | CTGTGTATACATTAAGTATCTTTC |
| SD | CCAGGAAGCATTTGGTACATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17294A-B6 | VGB6 | C57BL/6NTac | |
| 17294A-C3 | VGB6 | C57BL/6NTac | |
| 17294A-F3 | VGB6 | C57BL/6NTac | |
