| Gene | Actr1b |
| Chromosome | 1 |
| Deletion Start | 36,709,850 |
| Deletion End | 36,700,072 |
| Deletion Size | 9,779 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 36,709,587 | 36,709,608 | GGATCGCTGGGCAAGTTAAGAC |
| TUP | 36,709,563 | 36,709,585 | CCTGGCCCTTTGCTTGGTGGTAA |
| TUR | 36,709,512 | 36,709,535 | GCAGTGTGCAAGAAGTCAAATGTC |
| TDF | 36,700,395 | 36,700,415 | GGTGGCAGCCGTATGCTTATC |
| TDP | 36,700,362 | 36,700,390 | TCTTTGCTCCTAGATCTCTGCTCCTCAGG |
| TDR | 36,700,322 | 36,700,342 | TGAGCCCAATCTCACCCAATC |
PCR Primers
| Primer | Sequence |
| SU | CTACCTTGGCAACGCCTG |
| SD | CAAACACCACTGTGCCTCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17297A-A1 | VGB6 | C57BL/6NTac | |
| 17297A-D10 | VGB6 | C57BL/6NTac | |
| 17297A-F11 | VGB6 | C57BL/6NTac | |
