| Gene | Adss |
| Chromosome | 1 |
| Deletion Start | 177,796,391 |
| Deletion End | 177,764,238 |
| Deletion Size | 32,154 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 177,795,865 | 177,795,888 | GCACTGCATTACCTACAGGCTTAG |
| TUP | 177,795,841 | 177,795,863 | CACGCAGAACCTCGCCTCCATCG |
| TUR | 177,795,813 | 177,795,835 | AGCGGATTCTGCTATCAACAAAG |
| TDF | 177,764,391 | 177,764,414 | TGCTTTGATCTGAATGTGGGAGAG |
| TDP | 177,764,362 | 177,764,389 | TGGGAGTTTAGCTCTTGCCTAGCAGTAC |
| TDR | 177,764,338 | 177,764,360 | TCACACTTGGGTAAGGAAAGGAC |
PCR Primers
| Primer | Sequence |
| SU | GGATGGGGTGTGGCCTCC |
| SD | CTGGAAGTTCTAGATGTCAACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17298A-B11 | VGB6 | C57BL/6NTac | |
| 17298A-D2 | VGB6 | C57BL/6NTac | |
| 17298A-E7 | VGB6 | C57BL/6NTac | |
| 17298A-H9 | VGB6 | C57BL/6NTac | |
