| Gene | Afap1 |
| Chromosome | 5 |
| Deletion Start | 35,962,282 |
| Deletion End | 35,935,557 |
| Deletion Size | 26,726 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,935,638 | 35,935,658 | GCTGTGCTCACCAACATCCTG |
| TUP | 35,935,659 | 35,935,681 | CTGCGCCTGCAGTCATCCAAGGG |
| TUR | 35,935,704 | 35,935,726 | GAATTTGGAACCCTGGGAATCTG |
| TDF | 35,961,954 | 35,961,978 | CAGTGCATGTGTTCAGTTTATTGTC |
| TDP | 35,961,979 | 35,962,002 | TAGCCAGGGTGTCCCTCATGCCTG |
| TDR | 35,962,004 | 35,962,026 | TGAAAGGATTGAGGTCTGAGGTG |
PCR Primers
| Primer | Sequence |
| SU | TCACCCACTGACATGGAATG |
| SD | ACAAGACTATAACCCTGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17299A-D11 | VGB6 | C57BL/6NTac | |
| 17299A-F11 | VGB6 | C57BL/6NTac | |
| 17299A-H10 | VGB6 | C57BL/6NTac | |
