Gene | Afap1 |
Chromosome | 5 |
Deletion Start | 35,962,282 |
Deletion End | 35,935,557 |
Deletion Size | 26,726 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 35,935,638 | 35,935,658 | GCTGTGCTCACCAACATCCTG |
TUP | 35,935,659 | 35,935,681 | CTGCGCCTGCAGTCATCCAAGGG |
TUR | 35,935,704 | 35,935,726 | GAATTTGGAACCCTGGGAATCTG |
TDF | 35,961,954 | 35,961,978 | CAGTGCATGTGTTCAGTTTATTGTC |
TDP | 35,961,979 | 35,962,002 | TAGCCAGGGTGTCCCTCATGCCTG |
TDR | 35,962,004 | 35,962,026 | TGAAAGGATTGAGGTCTGAGGTG |
PCR Primers
Primer | Sequence |
SU | TCACCCACTGACATGGAATG |
SD | ACAAGACTATAACCCTGCAC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 89 bp | + | + | - |
TDF | TDR | 73 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
17299A-D11 | VGB6 | C57BL/6NTac | |
17299A-F11 | VGB6 | C57BL/6NTac | |
17299A-H10 | VGB6 | C57BL/6NTac | |