17299   Afap1     Status: ES cell colonies screened / QC positives

GeneAfap1
Chromosome5
Deletion Start35,962,282
Deletion End35,935,557
Deletion Size26,726
Genome Build38
MGI IDMGI:1917542
NCBI Gene ID70292
BAC ID312B8
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF35,935,63835,935,658GCTGTGCTCACCAACATCCTG
TUP35,935,65935,935,681CTGCGCCTGCAGTCATCCAAGGG
TUR35,935,70435,935,726GAATTTGGAACCCTGGGAATCTG
TDF35,961,95435,961,978CAGTGCATGTGTTCAGTTTATTGTC
TDP35,961,97935,962,002TAGCCAGGGTGTCCCTCATGCCTG
TDR35,962,00435,962,026TGAAAGGATTGAGGTCTGAGGTG

PCR Primers

PrimerSequence
SUTCACCCACTGACATGGAATG
SDACAAGACTATAACCCTGCAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR89 bp++-
TDFTDR73 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17299A-D11VGB6C57BL/6NTac
17299A-F11VGB6C57BL/6NTac
17299A-H10VGB6C57BL/6NTac