Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

17300 Agpat3 Status: ES cell colonies screened / QC positives

Gene	Agpat3
Chromosome	10
Deletion Start	78,288,021
Deletion End	78,273,615
Deletion Size	14,407
Genome Build	38

MGI ID	MGI:1336186
NCBI Gene ID	28169
BAC ID	423I19
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 10

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	78,287,685	78,287,705	TCTGGTTCCCTGAGGTCATAC
TUP	78,287,659	78,287,682	TTCCAGGGTCATGTGAACAGGTGC
TUR	78,287,628	78,287,649	CATGTGGTTGAGCCTGGTAAAG
TDF	78,274,064	78,274,085	GTTCGTGGGAGCAGGTAAAGAG
TDP	78,274,037	78,274,055	TGCCCGCTCACATGCTGGG
TDR	78,274,018	78,274,036	GTATGGGTGGCCCAGTGAC

PCR Primers

Primer	Sequence
SU	CCATTCATTCAGCCTTCCTG
SD	GAAGCTAAGGTGCCAAGCAG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	bp	-	+	+
TUF	TUR	78 bp	+	+	-
TDF	TDR	68 bp	+	+	-
NeoFwd	SD	bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
17300A-A10	VGB6	C57BL/6NTac
17300A-H10	VGB6	C57BL/6NTac
17300A-F4	VGB6	C57BL/6NTac
17300A-D12	VGB6	C57BL/6NTac