| Gene | Aif1l |
| Chromosome | 2 |
| Deletion Start | 31,971,068 |
| Deletion End | 31,950,354 |
| Deletion Size | 20,715 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,951,146 | 31,951,164 | GTCAGGGCCTCCTGGATTG |
| TUP | 31,951,166 | 31,951,188 | CAAAGCACAGAGGTGGCACAGCC |
| TUR | 31,951,214 | 31,951,237 | GAGACACCAGTCACATCTGAACAC |
| TDF | 31,970,758 | 31,970,780 | TCTGTGACTTAGGATCACCAACC |
| TDP | 31,970,784 | 31,970,806 | CCCTTCCACACAGCTAGCCCAGG |
| TDR | 31,970,809 | 31,970,829 | TGCAGCGTCCTCAACAGAATG |
PCR Primers
| Primer | Sequence |
| SU | CCGTATTTGCACCAACACAC |
| SD | TGTCGCTGAGAATGGACAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17302A-A8 | VGB6 | C57BL/6NTac | |
| 17302A-C9 | VGB6 | C57BL/6NTac | |
| 17302A-F3 | VGB6 | C57BL/6NTac | |
| 17302A-C11 | VGB6 | C57BL/6NTac | |
