| Gene | Alpl |
| Chromosome | 4 |
| Deletion Start | 137,758,629 |
| Deletion End | 137,742,365 |
| Deletion Size | 16,265 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,758,074 | 137,758,096 | AAAGTCACCACCCAGATCAGAAG |
| TUP | 137,758,049 | 137,758,072 | ACATTCCTAGGCACAGGCCAGGCC |
| TUR | 137,758,028 | 137,758,047 | TGGGAAAGGCACTGAGAAAC |
| TDF | 137,742,575 | 137,742,595 | GGCGTCCATGAGCAGAACTAC |
| TDP | 137,742,548 | 137,742,570 | CCCATGTGATGGCGTATGCCTCC |
| TDR | 137,742,514 | 137,742,532 | CCCAGGCACAGTGGTCAAG |
PCR Primers
| Primer | Sequence |
| SU | ACAGTTCAGCCTGAGAAGCC |
| SD | CTGGGAGTCTCATCCTGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17306A-A6 | VGB6 | C57BL/6NTac | |
| 17306A-C1 | VGB6 | C57BL/6NTac | |
| 17306A-G2 | VGB6 | C57BL/6NTac | |
| 17306A-F4 | VGB6 | C57BL/6NTac | |
| 17306A-C7 | VGB6 | C57BL/6NTac | |
