| Gene | Anks4b |
| Chromosome | 7 |
| Deletion Start | 120,183,092 |
| Deletion End | 120,173,922 |
| Deletion Size | 9,171 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 120,174,011 | 120,174,029 | TGGCATGACACCCACTCTC |
| TUP | 120,174,031 | 120,174,048 | TGGCAGCCTACCACGGCA |
| TUR | 120,174,076 | 120,174,098 | AACAGGAATCACTCACCCTCTAC |
| TDF | 120,182,970 | 120,182,989 | GGAAGCAGGTGCTCCAACAG |
| TDP | 120,182,990 | 120,183,013 | CCTGGGCAGTTGGTTGACACCAGC |
| TDR | 120,183,057 | 120,183,078 | TCCAGGAGAACTCAGCATGAAC |
PCR Primers
| Primer | Sequence |
| SU | TGCTTCACCACACTCATGAC |
| SD | CCTACCTGCTACACGTCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17309A-F10 | VGB6 | C57BL/6NTac | |
| 17309A-C12 | VGB6 | C57BL/6NTac | |
| 17309A-E6 | VGB6 | C57BL/6NTac | |
| 17309A-E9 | VGB6 | C57BL/6NTac | |
| 17309A-F11 | VGB6 | C57BL/6NTac | |
| 17309A-C9 | VGB6 | C57BL/6NTac | |
