17310   Ap1s2     Status: ES cell colonies screened / QC positives

GeneAp1s2
ChromosomeX
Deletion Start163,926,965
Deletion End163,911,332
Deletion Size15,634
Genome Build38
MGI IDMGI:1889383
NCBI Gene ID108012
BAC ID194E17
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF163,911,347163,911,365TTTAGTCGCCAGGGAAAGC
TUP163,911,367163,911,392TCGACTGCAGAAATGGTATGTCCCAC
TUR163,911,460163,911,480CACTCAAGGAAGCTGCACATC
TDF163,926,036163,926,054CAAAGGTGCCCACAGGAAG
TDP163,926,056163,926,085TCAAGATAGTAGACCTAGTTGTGCAGGGTC
TDR163,926,090163,926,112TGGTACAGCCCATCACAACATAC

PCR Primers

PrimerSequence
SUCACACTCTTCCACACTTACC
SDAATGGACCTTACAGCATCCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR134 bp++-
TDFTDR77 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17310A-A9VGB6C57BL/6NTac
17310A-F6VGB6C57BL/6NTac
17310A-G5VGB6C57BL/6NTac
17310A-G8VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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