| Gene | Arl1 |
| Chromosome | 10 |
| Deletion Start | 88,743,071 |
| Deletion End | 88,731,564 |
| Deletion Size | 11,508 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,731,900 | 88,731,917 | ATCCTTGCGCTGCTCCTG |
| TUP | 88,731,919 | 88,731,941 | CTGCTCGCCCGCTGTAGTTAGCT |
| TUR | 88,731,958 | 88,731,982 | ACTTCACAATCCACCTAACTCCATC |
| TDF | 88,742,445 | 88,742,468 | TGGACAGTTACTATGAGGTTGAGG |
| TDP | 88,742,471 | 88,742,500 | AGCCTGGTTTACTTAGAAAGATCAAGGCCA |
| TDR | 88,742,515 | 88,742,535 | GGGATTGCACCAGAGTTTCAC |
PCR Primers
| Primer | Sequence |
| SU | AGTTGTGGCGTTCATTTCTG |
| SD | TTTATACAAACAAGATGCAACCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17311A-H9 | VGB6 | C57BL/6NTac | |
| 17311A-A4 | VGB6 | C57BL/6NTac | |
| 17311A-B12 | VGB6 | C57BL/6NTac | |
| 17311A-F3 | VGB6 | C57BL/6NTac | |
| 17311A-E2 | VGB6 | C57BL/6NTac | |
| 17311A-C8 | VGB6 | C57BL/6NTac | |
