17312   Arrdc1     Status: ES cell colonies screened / QC positives

GeneArrdc1
Chromosome2
Deletion Start24,935,142
Deletion End24,925,594
Deletion Size9,549
Genome Build38
MGI IDMGI:2446136
NCBI Gene ID215705
BAC ID260H22
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF24,934,75224,934,770GTCTGTGCAAGGGCCTAAG
TUP24,934,72124,934,745TTCATTCACTATGCCCAGGCAACCC
TUR24,934,67524,934,699ACAACTGAACAAGGGAAGACTGAAG
TDF24,926,34424,926,364CGTGCTCAGTGGCAAGAACAG
TDP24,926,32424,926,343ATCCTGGTGCCTGCCCTGCC
TDR24,926,26024,926,284GCAACACCAAACCTGAAGGTAATAG

PCR Primers

PrimerSequence
SUAGGACTGGCCTGTTGTGAAC
SDGGTTCAAATGAGGAGCTTGC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR96 bp++-
TDFTDR105 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17312A-G6VGB6C57BL/6NTac
17312A-C11VGB6C57BL/6NTac
17312A-C9VGB6C57BL/6NTac
17312A-A10VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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