| Gene | Atp5f1 |
| Chromosome | 3 |
| Deletion Start | 105,959,184 |
| Deletion End | 105,942,964 |
| Deletion Size | 16,221 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,958,410 | 105,958,434 | CCATCCTCTAATCCGTCAGTAAGAC |
| TUP | 105,958,382 | 105,958,409 | TCAACTCGTGAGGATGCTTTGAGTCTGA |
| TUR | 105,958,347 | 105,958,368 | GCGGCTTCATGCTGTAACAATG |
| TDF | 105,943,292 | 105,943,316 | GTAGGTTTTGCTCATAAAAGCTGTG |
| TDP | 105,943,267 | 105,943,289 | CCAGGAAATTTTAGCTCACAGGA |
| TDR | 105,943,238 | 105,943,259 | CTGGGAGTGAGTTCCAGGATAG |
PCR Primers
| Primer | Sequence |
| SU | AGACTCGACGAGAGACCCTG |
| SD | AATTTCAGTTGGGCAGCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17314A-B6 | VGB6 | C57BL/6NTac | |
| 17314A-C5 | VGB6 | C57BL/6NTac | |
| 17314A-C6 | VGB6 | C57BL/6NTac | |
| 17314A-D4 | VGB6 | C57BL/6NTac | |
| 17314A-D12 | VGB6 | C57BL/6NTac | |
| 17314A-E3 | VGB6 | C57BL/6NTac | |
