| Gene | Atp6v0d1 |
| Chromosome | 8 |
| Deletion Start | 105,565,909 |
| Deletion End | 105,539,326 |
| Deletion Size | 26,584 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,565,610 | 105,565,628 | GCCTTCAAGAACCGCAGTG |
| TUP | 105,565,588 | 105,565,608 | TTTACCTGGGCTCGGGTTGCC |
| TUR | 105,565,565 | 105,565,586 | AGGACTAGCTCGACGAGGTTAG |
| TDF | 105,539,966 | 105,539,987 | GGCTGTCCTAGAATCCACTTTG |
| TDP | 105,539,938 | 105,539,964 | AGACTAGGATGGCCTTGAACTCCCAGA |
| TDR | 105,539,918 | 105,539,936 | TGGTGGCACACACAGGTAG |
PCR Primers
| Primer | Sequence |
| SU | ACTCCAGAGTAACGCATGC |
| SD | AGGAAATGAAACTGGTAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17315A-A6 | VGB6 | C57BL/6NTac | |
| 17315A-D3 | VGB6 | C57BL/6NTac | |
| 17315A-F3 | VGB6 | C57BL/6NTac | |
| 17315A-E5 | VGB6 | C57BL/6NTac | |
