| Gene | BC017612 |
| Chromosome | 9 |
| Deletion Start | 15,544,804 |
| Deletion End | 15,544,623 |
| Deletion Size | 182 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 15,544,629 | 15,544,652 | CTCAAAGGGAAGCCAAAGAAAGAG |
| TUP | 15,544,654 | 15,544,677 | CCTCCAAGGACAAGAAGGAGCGGA |
| TUR | 15,544,740 | 15,544,761 | ACACGACAATGAGGAGCACCAC |
| TDF | 15,544,676 | 15,544,694 | GAAGCAGGCCATGCAGGAG |
| TDP | 15,544,696 | 15,544,717 | CGCGGCAGCAGATCACCACTGT |
| TDR | 15,544,755 | 15,544,775 | GGCCACATACACAAACACGAC |
PCR Primers
| Primer | Sequence |
| SU | GGCCAGAGCAGTGTGTTAGG |
| SD | AAAGTTGTGAAGTGGAGCCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 133 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17317A-B12 | VGB6 | C57BL/6NTac | |
| 17317A-C2 | VGB6 | C57BL/6NTac | |
| 17317A-D5 | VGB6 | C57BL/6NTac | |
| 17317A-E12 | VGB6 | C57BL/6NTac | |
