17318   BC055324     Status: ES cell colonies screened / QC positives

GeneBC055324
Chromosome1
Deletion Start163,994,717
Deletion End163,954,127
Deletion Size40,591
Genome Build38
MGI IDMGI:3590554
NCBI Gene ID381306
BAC ID126L24
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 1

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF163,994,354163,994,372TCGGTCGCACTTGCCTAAG
TUP163,994,323163,994,345ATGCCCGCGCTGGTGCTCAGTAT
TUR163,994,298163,994,319CCTTCAGCTCTCACGCTTCATC
TDF163,954,516163,954,535CATTGGGAACCGTTGCACAG
TDP163,954,490163,954,515TGCGACTTCTTGTCTGCCACATACAA
TDR163,954,467163,954,489GCTACTACTCTCCACGTCCTAAC

PCR Primers

PrimerSequence
SUCCTATTCTCGAACGCACCTC
SDCAGTCTGTCCCTCTCCTTCG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR75 bp++-
TDFTDR69 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
17318A-B4VGB6C57BL/6NTac
17318C-G4VGB6C57BL/6NTac
17318C-H2VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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