| Gene | C1rl |
| Chromosome | 6 |
| Deletion Start | 124,507,160 |
| Deletion End | 124,493,154 |
| Deletion Size | 14,007 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,493,195 | 124,493,215 | ATGGAGTTCTCCCACCACAAG |
| TUP | 124,493,217 | 124,493,243 | TGCATGAGCAAGATGTGAGTTTGCACC |
| TUR | 124,493,282 | 124,493,302 | ATGTCCTGCCTTCCTCCCTTC |
| TDF | 124,507,062 | 124,507,081 | CCTTTGCTGCCCTCCTTCTG |
| TDP | 124,507,083 | 124,507,105 | TCTGCAGGGACACTTAGCTGCCC |
| TDR | 124,507,115 | 124,507,137 | CATCCTGTCTGTCCTTCCACTTC |
PCR Primers
| Primer | Sequence |
| SU | TGCTAGAGAGGAGGCAGAGC |
| SD | TCTGAGACTTCTGGAGAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17320A-D1 | VGB6 | C57BL/6NTac | |
| 17320A-D3 | VGB6 | C57BL/6NTac | |
| 17320A-H4 | VGB6 | C57BL/6NTac | |
| 17320A-H5 | VGB6 | C57BL/6NTac | |
| 17320A-H6 | VGB6 | C57BL/6NTac | |
| 17320A-F7 | VGB6 | C57BL/6NTac | |
