| Gene | Cars |
| Chromosome | 7 |
| Deletion Start | 143,600,003 |
| Deletion End | 143,570,547 |
| Deletion Size | 29,457 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,599,520 | 143,599,539 | ACTGGAGTGGGCAGAGATGG |
| TUP | 143,599,489 | 143,599,514 | CAGGTGAGTAGTATCCAGTCCAGGGC |
| TUR | 143,599,456 | 143,599,475 | CCACAGGCTTACCGGAGAAC |
| TDF | 143,570,748 | 143,570,769 | CTGGTGCAGTGAGGTTCAGAGG |
| TDP | 143,570,711 | 143,570,739 | TCATTTGCTTCAGTAGTAAAGGCCTGGGC |
| TDR | 143,570,688 | 143,570,708 | GCCCTGCTGACCATTCCAAAC |
PCR Primers
| Primer | Sequence |
| SU | TCTCCTCCAGTCGCTGAGTC |
| SD | CCACTTCTCTTAAGTGGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 17321A-A4 | VGB6 | C57BL/6NTac | |
| 17321A-F6 | VGB6 | C57BL/6NTac | |
| 17321A-B9 | VGB6 | C57BL/6NTac | |
| 17321A-B10 | VGB6 | C57BL/6NTac | |
| 17321A-E1 | VGB6 | C57BL/6NTac | |
| 17321A-B1 | VGB6 | C57BL/6NTac | |
